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Bi, Sai; Li, Li; Zhang, Shusheng Triggered Polycatenated DNA Scaffolds for DNA Sensors and Aptasensors by a Combination of Rolling Circle Amplification and DNAzyme Amplification //ANALYTICAL CHEMISTRY.- 2010, Vol.82, Iss.22, P.9447-9454.

The concept of triggered polycatenated DNA scaffolds has been elegantly introduced into ultrasensitive biosensing applications by a combination of rolling circle amplification (RCA) and DNAzyme amplification. As compared to traditional methods in which one target could only initiate the formation of one circular template for RCA reaction, in the present study two species of linear single-stranded DNA (ssDNA) monomers are self-assembled into mechanically interlocked polycatenated nanostructures on capture probe-tagged magnetic nanoparticles (MNPs) only upon the introduction of one base mutant DNA sequence as initiator for single-nucleotide polymorphisms (SNPs) analysis. The resultant topologically polycatenated DNA ladder is further available for RCA process by using the serially ligated circular DNA as template for the synthesis of hemin/G-quadruplex HRP-mimicking DNAzyme chains, which act as biocatalytic labels for the luminol-H(2)O(2) chemiluminescence (CL) system. Notably, the problem of high background induced by excess hemin itself is circumvented by immobilizing the biotinylated RCA products on streptavidin-modified MNPs via biotin-streptavidin interaction. Similarly, a universal strategy is contrived by substitutedly employing aptamer as initiator for the construction of polycatenated DNA scaffolds to accomplish ultrasensitive detection of proteins based on structure-switching of aptamer upon target binding, which is demonstrated by using thrombin as a model analyte in this study. Overall, with two successive amplification steps and one magnetic separation procedure, this flexible biosensing system exhibits not only high sensitivity and specificity with the detection limits of SNPs and thrombin as low as 71 aM and 6.6 pM, respectively, but also excellent performance in real human serum assay with no PCR preamplification for SNPs assay. Given the unique and attractive characteristics, this study illustrates the potential of DNA nanotechnology in bioanalytical applications for both fundamental and practical research

Bredenbeck, Jens; Ghosh, Avishek; Nienhuys, Han-Kwang; et al. Interface-Specific Ultrafast Two-Dimensional Vibrational Spectroscopy//ACCOUNTS OF CHEMICAL RESEARCH.-2009, Vol.42, Iss.9, P.1332-1342.

Surfaces and interfaces are omnipresent in nature. They are not just the place where two bulk media meet. Surfaces and interfaces play key roles in a diversity of fields ranging from heterogeneous catalysis and membrane biology to nanotechnology. They are the site of important dynamical processes, such as transport phenomena, energy transfer, molecular interactions, as well as chemical reactions. Tools to study molecular structure and dynamics that can be applied to the delicate molecular layers at surfaces and interfaces are thus highly desirable. The advent of multidimensional optical spectroscopies, which are the focus of a special issue of Accounts of Chemical Research, and in particular of two-dimensional infrared (2D-IR) spectroscopy has been a breakthrough in the investigation of ultrafast molecular dynamics in bulk media. This Account reviews our recent work extending 2D-IR spectroscopy to make it surface-specific, allowing us to reveal the structure and dynamics of specifically interfacial molecules.




A coral-like carbon material (Coral-C) is synthesized by growing curled carbon nanotubes (CNTs) onto carbon black (Lamp Black) to incorporate the unique structures and properties of the two nanostructured carbons, CNTs and carbon spheres. This Coral-C, having a good electronic conductivity, is used as a supporting material for Pt nanoatalyst for application in fuel cell electrodes. The Pt nanoparticles, being synthesized by a ligand exchange method, are stabilized on Coral-C though an enhanced deposition proess with poly(oxyproplyene)diamines. The Coral-C supported Pt catalyst shows excellent electrohemical active area (102.5 m2 g-1 Pt), good catalytic activity toward methanol oxidation (1.5 times higher than E-TEK Pt/C), and good power output in single DMFC (1.3 times better than E-TEK Pt/C), which could be attributed to the unique nanostructure of the catalyst: high conductivity of the surface accessible support and highly distributed Pt nanoparticles. The successful advancement in this coral-like nanostructure design for fuel cell catalyst presents a significant achievement in both the scientific and engineering fields. © 2010 American Chemical Society.

Chen, Anna H.; Silver, Pamela A. Designing biological compartmentalization //TRENDS IN CELL BIOLOGY .-2012, V.22, Iss. 12, P.662-670.

Intracellular organization is a key factor in cell metabolism. Cells have evolved various organizational systems to solve the challenges of toxic pathway intermediates, competing metabolic reactions, and slow turnover rates. Inspired by nature, synthetic biologists have utilized proteins, nucleic acids, and lipids to construct synthetic organizational systems that mimic natural systems. Many of these systems have been applied to metabolic pathways and shown to significantly increase the production of industrially and commercially important chemicals. Further engineering and characterization of synthetic organizational systems will allow us to better understand native cellular strategies of spatial organization. Here, we discuss recent advances and ongoing efforts in designing and characterizing synthetic compartmentalization systems to mimic natural strategies and increase metabolic yields of engineered pathways.

Chen, Jun; Zhou, Jianhong; Sanders, Claire K.; et al. A surface display yeast two-hybrid screening system for high-throughput protein interactome mapping //ANALYTICAL BIOCHEMISTRY -2009, Volume: 390 Issue: 1 Pages: 29-37

Despite the wide acceptance of yeast two-hybrid (Y2H) system for protein-protein interaction analysis and discovery, conventional Y2H assays are not well suited for high-throughput screening of the protein interaction network ("interactome") on a genomic scale due to several limitations, including labor-intensive agar plating and colony selection methods associated with the use of nutrient selection markers, complicated reporter analysis methods associated with the use of LacZ enzyme reporters, and incompatibility of the liquid handling robots. We recently reported a robust liquid culture Y2H system based on quantitative analysis of yeast-enhanced green fluorescent protein (yEGFP) reporters that greatly increased the analysis throughput and compatibility with liquid handling robots. To further advance its utility in high-throughput complementary DNA (cDNA) library screening, we report the development of a novel surface display Y2H (sdY2H) library screening system with uniquely integrated surface display hemagglutination (sdHA) antigen and yEGFP reporters. By introduction of a surface reporter sdHA into the yEGFP-based Y2H system, positive Y2H targets are quickly isolated from library cells by a simple magnetic separation Without a large plating effort. Moreover, the simultaneous scoring of multiple reporters, including sdHA, yEGFP, and conventional nutrient markers, greatly increased the specificity of the Y2H assay. The feasibility of the sdY2H assay on large cDNA library screening was demonstrated by the successful recovery of positive P53/T interaction pairs at a target-to-background ratio of 1:1,000,000. Together with the massive parallel DNA sequencing technology, it may provide a powerful proteomic tool for high-throughput interactome mapping on a genomic scale

Gao, Jinhao; Gu, Hongwei; Xu, Bing. Multifunctional Magnetic Nanoparticles: Design, Synthesis, and Biomedical Applications // Accounts of Chemical Research(USA).- 2009 г., v. 42, N 8,P.1097-1107.

The combination of nanotechnology and molecular biology has developed into an emerging research area: nano-biotechnology. Magnetic nanoparticles are well-established nanomaterials that offer controlled size, ability to be manipulated externally, and enhancement of contrast in magnetic resonance imaging (MRI). As a result, these nanoparticles could have many applications in biology and medicine, including protein purification, drug delivery, and medical imaging. Because of the potential benefits of multimodal functionality in biomedical applications, researchers would like to design and fabricate multifunctional magnetic nanoparticles. Currently, there are two strategies to fabricate magnetic nanoparticle-based multifunctional nanostructures. The first, molecular functionalization, involves attaching antibodies, proteins, and dyes to the magnetic nanoparticles. The other method integrates the magnetic nanoparticles with other functional nanocomponents, such as quantum dots (QDs) or metallic nanoparticles. Because they can exhibit several features synergistically and deliver more than one function simultaneously, such multifunctional magnetic nanoparticles could have unique advantages in biomedical applications.

Godula, Kamil; Umbel, Marissa L.; Rabuka, David; et al. Control of the Molecular Orientation of Membrane-Anchored Biomimetic Glycopolymers// JOURNAL OF THE AMERICAN CHEMICAL SOCIETY -2009, Volume: 131 Issue: 29 Pages: 10263-10268 .

Quantifying and controlling the orientation of surface-bound macromolecules is crucial to a wide range of processes in areas as diverse as biology, materials science, and nanotechnology. Methods capable of directing orientation, as well as an understanding of the underlying physical mechanisms are, however, lacking. In this paper, we describe experiments in which the conformations of structurally well-defined polymers anchored to fluid lipid membranes were probed using Fluorescence Interference Contrast Microscopy (FLIC), an optical technique that provides topographic information with few-nanometer precision. The novel rodlike polymers mimic the architecture of mucin glycoproteins and feature a phospholipid tail for membrane incorporation and a fluorescent optical probe for FLIC imaging situated at the opposite termini of the densely glycosylated polymeric backbones. We find that the orientation of the rigid, approximately 30 nm long glycopolymers depends profoundly on the properties of the optical reporter. Molecules terminated with Alexa Fluor 488 projected away from the lipid bilayer by 11 +/- 1 nm, consistent with entropy-dominated sampling of the membrane-proximal space. Molecules terminated with Texas Red lie flat at the membrane (height, 0 +/- 2 nm), implying that interactions between Texas Red and the bilayer dominate the polymers' free energy. These results demonstrate the design of macromolecules with specific orientational preferences, as well as nanometer-scale measurement of their orientation. Importantly, they reveal that seemingly minute changes in molecular structure, in this case fluorophores that comprise only 2% of the total molecular weight, can significantly alter the molecule's presentation to the surrounding environment

Grabow, Wade W.; Zhuang, Zhuoyun; Swank, Zoe N.; et al. The Right Angle (RA) Motif: A Prevalent Ribosomal RNA Structural Pattern Found in Group I Introns // JOURNAL OF MOLECULAR BIOLOGY.- 2012, Volume: 424 Issue: 1-2 Pages: 54-67.

The right angle (RA) motif, previously identified in the ribosome and used as a structural module for nano-construction, is a recurrent structural motif of 13 nucleotides that establishes a 90 degrees bend between two adjacent helices. Comparative sequence analysis was used to explore the sequence space of the RA motif within ribosomal RNAs in order to define its canonical sequence space signature. We investigated the sequence constraints associated with the RA signature using several artificial self-assembly systems. Thermodynamic and topological investigations of sequence variants associated with the RA motif in both minimal and expanded structural contexts reveal that the presence of a helix at the 3' end of the RA motif increases the thermodynamic stability and rigidity of the resulting three-helix junction domain. A search for the RA in naturally occurring RNAs as well as its experimental characterization led to the identification of the RA in groups IC1 and ID intron ribozymes, where it is suggested to play an integral role in stabilizing peripheral structural domains.

Hariadi, Rizal F.; Cale, Mario; Sivaramakrishnan, Sivaraj. Myosin lever arm directs collective motion on cellular actin network //PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA - 2014, Volume: 111 Issue: 11 Pages: 4091-4096 .

The molecular motor myosin teams up to drive muscle contraction, membrane traffic, and cell division in biological cells. Myosin function in cells emerges from the interaction of multiple motors tethered to a scaffold, with surrounding actin filaments organized into 3D networks. Despite the importance of myosin function, the influence of intermotor interactions on collective motion remains poorly understood. In this study, we used precisely engineered myosin assemblies to examine emergence in collective myosin movement. We report that tethering multiple myosin VI motors, but not myosin V motors, modifies their movement trajectories on keratocyte actin networks. Single myosin V and VI dimers display similar skewed trajectories, albeit in opposite directions, when traversing the keratocyte actin network. In contrast, tethering myosin VI motors, but not myosin V motors, progressively straightens the trajectories with increasing myosin number. Trajectory shape of multimotor scaffolds positively correlates with the stiffness of the myosin lever arm. Swapping the flexible myosin VI lever arm for the relatively rigid myosin V lever increases trajectory skewness, and vice versa. A simplified model of coupled motor movement demonstrates that the differences in flexural rigidity of the two myosin lever arms is sufficient to account for the differences in observed behavior of groups of myosin V and VI motors. In accordance with this model trajectory, shapes for scaffolds containing both myosin V and VI are dominated by the myosin with a stiffer lever arm. Our findings suggest that structural features unique to each myosin type may confer selective advantages in cellular functions.

Judith T.M.L. Paridaen, Michaela Wilsch-Brauninger, Wieland B. Huttner. Asymmetric Inheritance of Centrosome-Associated Primary Cilium Membrane Directs Ciliogenesis after Cell Division.//Cell.-2013, Volume 155, Issue 2, Pages 333-344.

Primary cilia are key sensory organelles that are thought to be disassembled prior to mitosis. Inheritance of the mother centriole, which nucleates the primary cilium, in relation to asymmetric daughter cell behavior has previously been studied. However, the fate of the ciliary membrane upon cell division is unknown. Here, we followed the ciliary membrane in dividing embryonic neocortical stem cells and cultured cells. Ciliary membrane attached to the mother centriole was endocytosed at mitosis onset, persisted through mitosis at one spindle pole, and was asymmetrically inherited by one daughter cell, which retained stem cell character. This daughter re-established a primary cilium harboring an activated signal transducer earlier than the noninheriting daughter. Centrosomal association of ciliary membrane in dividing neural stem cells decreased at late neurogenesis when these cells differentiate. Our data imply that centrosome-associated ciliary membrane acts as a determinant for the temporal-spatial control of ciliogenesis

Jung, Jangwook P.; Squirrell, Jayne M.; Lyons, Gary E.; et al. Imaging cardiac extracellular matrices: a blueprint for regeneration //TRENDS IN BIOTECHNOLOGY.- 2012, Volume: 30 Issue: 4 Pages: 233-240.

Once damaged, cardiac tissue does not readily repair and is therefore a primary target of regenerative therapies. One regenerative approach is the development of scaffolds that functionally mimic the cardiac extracellular matrix (ECM) to deliver stem cells or cardiac precursor populations to the heart. Technological advances in micro/nanotechnology, stem cell biology, biomaterials and tissue decellularization have propelled this promising approach forward. Surprisingly, technological advances in optical imaging methods have not been fully utilized in the field of cardiac regeneration. Here, we describe and provide examples to demonstrate how advanced imaging techniques could revolutionize how ECM-mimicking cardiac tissues are informed and evaluated.


The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold nanaoaggregates and 4-mercaptobenzoic acid (pMBA) attached as a reporter for monitoring changes in local pH of the cellular compartments of living NIH/3T3 cells. We show that SERS nanosensors enable the dynamics of local pH in individual live cells to be followed at subendosomal resolution in a timeline of cellular processes. This information is of basic interest for a better understanding of a broad range of physiological and metabolic processes as well as for a number of biotechnological applications.

Kshitiz; Kim, Deok-Ho; Beebe, David J.; et al. Micro- and nanoengineering for stem cell biology: the promise with a caution //TRENDS IN BIOTECHNOLOGY .- 2011, Volume: 29 Issue: 8 Pages: 399-408.

Current techniques used in stem cell research only crudely mimic the physiological complexity of the stem cell niches. Recent advances in the field of micro- and nanoengineering have brought an array of in vitro cell culture models that have enabled development of novel, highly precise and standardized tools that capture physiological details in a single platform, with greater control, consistency, and throughput. In this review, we describe the micro- and nanotechnology-driven modern toolkit for stem cell biologists to design novel experiments in more physiological microenvironments with increased precision and standardization, and caution them against potential challenges that the modern technologies might present.


RNA interference (RNAi) is an evolutionarily conserved sequence-specific post-transcriptional gene silencing pathway with wide-ranging applications in functional genomics, therapeutics, and biotechnology. Cationic liposome-small interfering RNA (CL-siRNA) complexes have emerged as vectors of choice for delivery of siRNA, which mediates RNAi. However, siRNA delivery by CL-siRNA complexes is often inefficient and accompanied by lipid toxicity. We report the development of CL-siRNA complexes with a novel cubic phase nanostructure, which exhibit efficient silencing at low toxicity. The inverse bicontinuous gyroid cubic nanostructure was unequivocally established from synchrotron X-ray scattering data, while fluorescence microscopy revealed colocalization of lipid and siRNA in complexes. We attribute the efficient silencing to enhanced fusion of complex and endosomal membranes, facilitated by the cubic phase membrane's positive Gaussian modulus, which may enable spontaneous formation of transient pores. The findings underscore the importance of understanding membrane-mediated interactions between CL-siRNA complex nanostructure and cell components in developing CL-based gene silencing vectors

Li H., Jiang B., Schaller R., Wu J., Jiao. ANTIREFLECTIVE PHOTOANODE MADE OF TIO2 NANOBELTS AND a Zn Nanowire Array //Journal of Physical Chemistry.C. Nanomaterials and Interfaces(USA).- 2010, V. 114, N 26, P.11375-11380.



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Liu, Yingting; Zhu, Fangqiang. Collective Diffusion Model for Ion Conduction through Microscopic Channels// BIOPHYSICAL JOURNAL .-2013, Vol.104,Iss.2 , P.368-376.

Ion conduction through microscopic channels is of central importance in both biology and nanotechnology. To better understand the current-voltage (I-V) dependence of ion channels, here we describe and prove a collective diffusion model that quantitatively relates the spontaneous ion permeation at equilibrium to the stationary ionic fluxes driven by small voltages. The model makes it possible to determine the channel conductance in the linear I-V range from equilibrium simulations without the application of a voltage. To validate the theory, we perform molecular-dynamics simulations on two channels-a conical-shaped nanopore and the transmembrane pore of an alpha-hemolysin-under both equilibrium and nonequilibrium conditions. The simulations reveal substantial couplings between the motions of cations and anions, which are effectively captured by the collective coordinate in the model. Although the two channels exhibit very different linear ranges in the I-V curves, in both cases the channel conductance at small voltages is in reasonable agreement with the prediction from the equilibrium simulation.

Maeda-Mamiya, Rui; Noiri, Eisei; Isobe, Hiroyuki; et al. In vivo gene delivery by cationic tetraamino fullerene.//PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA .- 2010, Vol.107, Iss.12, P.5339-5344.

Application of nanotechnology to medical biology has brought remarkable success. Water-soluble fullerenes are molecules with great potential for biological use because they can endow unique characteristics of amphipathic property and form a self-assembled structure by chemical modification. Effective gene delivery in vitro with tetra(piperazino) fullerene epoxide (TPFE) and its superiority to Lipofectin have been described in a previous report. For this study, we evaluated the efficacy of in vivo gene delivery by TPFE. Delivery of enhanced green fluorescent protein gene (EGFP) by TPFE on pregnant female ICR mice showed distinct organ selectivity compared with Lipofectin; moreover, higher gene expression by TPFE was found in liver and spleen, but not in the lung. No acute toxicity of TPFE was found for the liver and kidney, although Lipofectin significantly increased liver enzymes and blood urea nitrogen. In fetal tissues, neither TPFE nor Lipofectin induced EGFP gene expression. Delivery of insulin 2 gene to female C57/BL6 mice increased plasma insulin levels and reduced blood glucose concentrations, indicating the potential of TPFE-based gene delivery for clinical application. In conclusion, this study demonstrated effective gene delivery in vivo for the first time using a water-soluble fullerene.


Delivery vectors having targeting ligands provide an impending impact on cancer gene therapy. Our work focuses on folate mediated targeting induced by conjugating poly(ethylene glycol)-folate (PEG-FA) with arginine modified chitosan polymer having low molecular weight of 15. kDa and high degree of quaternization (ATFP15H). The ATFP15H derivative on condensation with plasmid DNA formed nanoparticles with core shell nanostructure. It also affirmed good buffering capacity. The derivative was found to protect DNA from DNase I degradation and also from disassembly in presence of negatively charged plasma proteins. It exhibited blood compatibility in terms of percentage hemolysis, erythrocyte aggregation and also by platelet activation. At a concentration of 10 ?g, the capability of the derivative to enhance cell growth at normal cell growing conditions was observed. The transfection efficiency was also found to be comparable to PEI when transfected in KB cell line, which over expressed the folate receptor (FR) in presence of 10% fetal bovine serum (FBS). On comparison with native chitosan and trimethylated chitosan, ATFP15H derivative exhibited high cellular uptake and nuclear localization due to the superior colloidal stability attained on conjugation with polyethylene glycol. This has been ascertained by flow cytometry and YOYO labeling of plasmid DNA.


Organic nanocrystals (NCs) are in the mesoscopic phase between a single molecule and the corresponding bulk crystals, and are expected to exhibit peculiar optical properties, depending on crystal size and shape. In the present Award Accounts, recent progress on hybridized organic NCs and ordered array structure of encapsulated organic NCs will be introduced in detail for optically functional materials toward next-generation organic device application. Hybrid material (or hybridization) is an important area in current material science. Our attention is now focused on coreshell type hybridized organic NCs, which seem to be the best suited nanostructure for providing novel optoelectronic properties and photonic function induced by coreshell interface interaction. On the other hand, it may be necessary to arrange and integrate organic NCs, including hybridized materials, on a substrate so as to receive and transmit input and output signals by electronically and/or optically accessing organic devices. Hence, encapsulations of organic NCs, patterned substrates, and tapered cell method have been employed suitably to fabricate and control ordered array structure of organic NCs on a substrate. Finally, the future scope in the relevant fields of optoelectronics and photonics will be discussed in brief

Oren, Ersin Emre; Notman, Rebecca; Kim, Il Won; et al. Probing the Molecular Mechanisms of Quartz-Binding Peptides // LANGMUIR.- 2010, V.26, Iss.13, P.11003-11009.

Understanding the mechanisms of biomineralization and the realization of biology-inspired inorganic materials formation largely depends on our ability to manipulate peptide/solid interfacial interactions. Material interfaces and biointerfaces are critical sites for bioinorganic synthesis, surface diffusion, and molecular recognition. Recently adapted biocombinatorial techniques permit the isolation of peptides recognizing inorganic solids that are used as molecular building blocks, for example, as synthesizers, linkers, and assemblers. Despite their ubiquitous utility in nanotechnology, biotechnology, and medicine, the fundamental mechanisms of molecular recognition of engineered peptides binding to inorganic surfaces remain largely unknown. To explore propensity rules connecting sequence, structure, and function that play key roles in peptide/solid interactions, we combine two different approaches: a statistical analysis that searches for highly enriched motifs among de novo designed peptides, and, atomistic simulations of three experimentally validated peptides. The two strong and one weak quartz-binding peptides were chosen for the simulations at the quartz (1 00) surface under aqueous conditions. Solution-based peptide structures were analyzed by circular dichroism measurements. Small and hydrophobic residues, such as Pro, play a key role at the interface by making close contact with the solid and hindering formation of intrapeptide hydrogen bonds. The high binding affinity of a peptide may be driven by a combination of favorable enthalpic and entropic effects, that is, a strong binder may possess a large number of possible binding configurations, many of which having relatively high binding energies. The results signify the role of the local molecular environment among the critical residues that participate in solid binding. The work herein describes molecular conformations inherent in material-specific peptides and provides fundamental insight into the atomistic understanding of peptide/solid interfaces


Spontaneous electrochemical synthesis of one-dimensional zinc oxide (1D ZnO) nanostructure from Zn anodes in an electrochemical cell is preliminarily demonstrated. By varying the discharged current up to 165 mA, a larger amount of Zn (OH)2 composites with few quantity of nucleation sites [Zn (OH)4] 2- are obtained to promote an anisotropic growth of 1D ZnO nanostructure. The morphology and the size-controlled 1D ZnO structure can achieve a larger scale than others by a inducing larger quantity of Zn (OH)2 near the nucleation site to promote the quick reaction. Such a reaction provides a strong and distinct narrow photoluminescent spectrum for the synthesized ZnO with (100) wurtzite structure with well crystallized and expanding grain. The room temperature photoluminescence and X-ray energy-dispersive spectra indicate that the composition-ratio varied ZnO with a zinc-rich condition as well as relatively low O/Zn composition ratio is preferably obtained in KOH aqueous solution at high discharged current. Our results elucidate that the significantly decreased defect-related luminescence and enhanced bandgap UV emission is controllable via the tunable discharged current and reactive time induced optimization on the quantity of (Zn (OH) 2) and [Zn (OH)4] 2- precursors

Petrakova, O. S.; Chernioglo, E. S.; Terskikh, V. V.; et al. The Use of Cellular Technologies in Treatment of Liver Pathologies //ACTA NATURAE.- 2012, Vol.4, Iss.3, P.18-33 .

Cell techniques find increasing application in modern clinical practice. The II and III phases of clinical trials are already under way for various cellular products used for the restoration of the functions of the cornea, larynx, skin, etc. However, the obtainment of functional cell types specific to different organs and tissues still remains a subject of laboratory research. Liver is one of the most important organs; the problems and prospects of cellular therapy for liver pathologies are currently being actively studied. Cellular therapy of liver pathologies is a complex multistage process requiring a thorough understanding of the molecular mechanisms occurring in liver cells during differentiation and regeneration. An analysis of the current cellular therapy for liver pathologies is presented, the use of various cell types is described, the main molecular mechanisms of hepatocyte differentiation are analyzed, and the challenges and prospects of cell therapy for liver disorders are discussed in this review

Raphael, Marc P.; Christodoulides, Joseph A.; Delehanty, James B.; et al. Quantitative LSPR Imaging for Biosensing with Single Nanostructure Resolution //BIOPHYSICAL JOURNAL .- 2013,Vol.104, Iss.1, P.30-36.

Localized surface plasmon resonance (LSPR) imaging has the potential to map complex spatio-temporal variations in analyte concentration, such as those produced by protein secretions from live cells. A fundamental roadblock to the realization of such applications is the challenge of calibrating a nanoscale sensor for quantitative analysis. Here, we introduce a new, to our knowledge, LSPR imaging and analysis technique that enables the calibration of hundreds of individual gold nanostructures in parallel. The calibration allowed us to map the fractional occupancy of surface-bound receptors at individual nanostructures with nanomolar sensitivity and a temporal resolution of 225 ms. As a demonstration of the technique's applicability to molecular and cell biology, the calibrated array was used for the quantitative LSPR imaging of anti-c-myc antibodies harvested from a cultured 9E10 hybridoma cell line without the need for further purification or processing

Strzelecki, J.; Dabrowski, M.; Strzelecka, J.; et al. AFM Investigation of Biological Nanostructures//ACTA PHYSICA POLONICA A.- 2012, Vol.122, Iss.2, P.329-332.

Nanostructures created by living organisms, optimized through millions of years of evolution, can be a valuable inspiration for nanotechnology. We employ atomic force microscopy to examine such structures in materials created by common organisms - caddisfly and diatoms. Caddisfly larvae are well known for their ability to spin silk, which serves as an "adhesive tape" to glue various materials and collect food in aqueous environment. Atomic: force microscopy imaging of caddisfly silk, performed for the first time by our team, has shown that its surface is patterned with 150 nm extensions - a feature related to its exceptional underwater sticking abilities. Results of force spectroscopy of protein structures found on the surface are also shown. A characteristic feature of diatoms is that they are encased within a unique silica cell wall called frustules, patterned with 200 nm pores, which allow cellular interaction with the environment. We perform atomic force microscopy imaging of frustules in living diatoms as well as adhesion measurements inside pores

Sun, Jiefang; Guo, Lei; Bao, Yi; et al. A simple, label-free AuNPs-based colorimetric ultra sensitive detection of nerve agents and highly toxic organophosphate pesticide //BIOSENSORS & BIOELECTRONICS .- 2011, Vol.28, Iss.1, P.152-157.

Here, a simple label-free colorimetric sensing method for organophosphate (OP) nerve agents and pesticide based on catalytic reaction of acetylcholine esterase (AChE) and the aggregation of lipoic acid (LA) capped AuNPs has been established, which is highly sensitive with a limit of detection (LOD) lowered to pM level. In this method, only the AChE hydrolysis product of acetylthiocholine (ATCh), i.e., cationic thiocholine (TCh) can induce the aggregation of LA capped AuNPs along with a distinct color change from red to steel-blue. When OPs as enzyme inhibitors exist, the generation of TCh can be suppressed and the color change of LA capped AuNPs is gradually diminished according to different concentrations of OPs. The feasibility of this method has been demonstrated by sensitive measurement of OP nerve agents and pesticide in a spiked fruit sample with reliable results. This distinct and rapid colorimetric response enables us to readily probe OPs without more technical demand

Tuleuova, Nazgul; Jones, Caroline N.; Yan, Jun; et al. Development of an Aptamer Beacon for Detection of Interferon-Gamma //ANALYTICAL CHEMISTRY.- 2010, Vol.82, Iss.5, P.1851-1857.

Traditional antibody-based affinity sensing strategies employ multiple reagents and washing steps and are unsuitable for real-time detection of analyte binding. Aptamers, on the other hand, may be designed to monitor binding events directly, in real-time, without the need for secondary labels. The goal of the present study was to design ail aptamer beacon for fluorescence resonance energy transfer (FRET)based detection of interferon-gamma (IFN-gamma)-an important inflammatory cytokine. Variants of DNA aptamer modified with biotin moieties and spacers were immobilized oil avidin-coated surfaces and characterized by surface plasmon resonance (SPR). The SPR studies showed that immobilization of aptamer via the 3' end resulted in the best binding IFN-gamma (K(d) = 3.44 nM). This optimal aptamer variant was then used to construct a beacon by hybridizing fluorophore-labeled aptamer with an antisense oligonucleotide strand carrying a quencher. SPR studies revealed that IFN-gamma binding with an aptamer beacon occurred within 15 min of analyte introduction-suggesting dynamic replacement of the quencher-complementary strand by IFN-gamma molecules. To further highlight biosensing applications, aptamer beacon molecules were immobilized inside microfluidic channels and challenged with varying concentration of analyte. Fluorescence microscopy revealed low fluorescence in the absence of analyte and high fluorescence after introduction of IFN-gamma. Importantly, unlike traditional antibody-based immunoassays, tire signal was observed directly upon binding of analyte without the need for multiple washing steps. The surface immobilized aptamer beacon had a linear range from 5 to 100 nM and a lower limit of detection of 5 nM IFN-gamma. In conclusion, we designed a FRET-based aptamer beacon for monitoring of an inflammatory cytokine-IFN-gamma. In the future, this biosensing strategy will be employed to monitor dynamics of cytokine production by the immune cells

Wang H., Bai Y., Zhang. CDS QUANTUM DOTS-SENSITIZED TIO2 NANOROD ARRAY ON TRANSPARENT CONDUCTIVE //Journal of Physical Chemistry.C. Nanomaterials and Interfaces(USA).- 2010, Vol.114, N 39, P.16451-16455.

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Wong, Ian Y.; Bhatia, Sangeeta N.; Toner, Mehmet. Nanotechnology: emerging tools for biology and medicine //GENES & DEVELOPMENT.- 2013, Vol.27, Iss.22, P.2397-2408.

Historically, biomedical research has been based on two paradigms. First, measurements of biological behaviors have been based on bulk assays that average over large populations. Second, these behaviors have then been crudely perturbed by systemic administration of therapeutic treatments. Nanotechnology has the potential to transform these paradigms by enabling exquisite structures comparable in size with biomolecules as well as unprecedented chemical and physical functionality at small length scales. Here, we review nanotechnology-based approaches for precisely measuring and perturbing living systems. Remarkably, nanotechnology can be used to characterize single molecules or cells at extraordinarily high throughput and deliver therapeutic payloads to specific locations as well as exhibit dynamic biomimetic behavior. These advances enable multimodal interfaces that may yield unexpected insights into systems biology as well as new therapeutic strategies for personalized medicine

Wong, Joyce Y.; McDonald, John; Taylor-Pinney, Micki; et al. Materials by design: Merging proteins and music.//NANO TODAY.- 2012, Vol.7, Iss.6, P.488-495.

Tailored materials with tunable properties are crucial for applications as biomaterials, for drug delivery, as functional coatings, or as lightweight composites. An emerging paradigm in designing such materials is the construction of hierarchical assemblies of simple building blocks into complex architectures with superior properties. We review this approach in a case study of silk, a genetically programmable and processable biomaterial, which, in its natural role serves as a versatile protein fiber with hierarchical organization to provide structural support, prey procurement or protection of eggs. Through an abstraction of knowledge from the physical system, silk, to a mathematical model using category theory, we describe how the mechanism of spinning fibers from proteins can be translated into music through a process that assigns a set of rules that governs the construction of the system. This technique allows one to express the structure, mechanisms and properties of the 'material' in a very different domain, 'music'. The integration of science and art through categorization of structure-property relationships presents a novel paradigm to create new bioinspired materials, through the translation of structures and mechanisms from distinct hierarchical systems and in the context of the limited number of building blocks that universally governs


Two types of silica nanoparticles having differing concentrations of ionizable surface groups are used to investigate the interplay between nanoparticle surface charge and solvent dielectric constant in nanostructure development during layer-by-layer assembly with a cationic polyacrylamide. Zeta (?) potential measurements are used to determine the extent of silanol dissociation with pH. For 19-nm-diameter X-Tec 3408 silica nanoparticles from Nano-X GmbH (NanoX), complete dissociation yields a ?-potential value of about -44. mV and occurs between pH 5 and 6 in 50% ethanol-in-water mixture by volume. By contrast, 65-nm-diameter polishing silica from Electron Microscopy Supply (EMS) has a ? potential that does not equilibrate even up to pH 7 with a value of -59. mV under otherwise similar solution conditions. The more negative zeta potential at a given pH is found to substantially reduce nanoparticle adsorption. This behavior is opposite that observed when the dielectric constant of the suspension is decreased, independent of particle size. Nanoparticle surface chemical heterogeneity is discussed as a plausible explanation for such seriously discrepant behavior and the effects on multilayer electrical contact resistance for proton-exchange membrane (PEM) fuel-cell coating applications are presented

Бусыгина Т.В., Елепов Б.С., Зибарева И.В., Лаврик О.Л., Шабурова Н.Н. Исследования Сибирского отделения РАН в области нанонауки и нанотехнологии: библиометрический анализ.//Химия в интересах устойчивого развития (ВАК)(RUS).- 2013 г., т. 21, N 4, Cтр. 463-473.

На основе библиометрического анализа публикаций СО РАН в области нанонауки и нанотехнологии за 2000-2011 г.г., представленных в БД Wos, Caplus, Scopus, Inspec и РИНЦ, выявлена их положительная динамика, определено преобладание статей и обзоров,причем англоязычных. Выявлены отечественные и зарубежные партнерские организации, идентифицированы наиболее продуктивные институты и сотрудники СО РАН.

Румянцев К.А.,Шеметов А.А.,Набиев И.Р. и др. ВЗАИМОДЕЙСТВИЕ БЕЛКОВ И ПЕПТИДОВ С НАНОЧАСТИЦАМИ: СТРУКТУРНЫЕ И ФУНКЦИОНАЛЬНЫЕ АСПЕКТЫ//Российские нанотехнологии (ВАК)(RUS).- 2013 г., т. 8, N 11, Cтр. 18-34.

Последние достижения в области нанотехнологии открывают широкие возможности для применения техногенных наночастиц в биологии и медицине. Благодаря своему размеру наночастицы способны прогикнуть практически во все органы, ткани, клетки и клеточные органеллы.

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